Advanced GCxGC-MS Strategy for Pesticide Analysis in a Highly Complex Chili Pepper Matrix

Michal Stupák, PhD, Assistant Professor at the University of Chemistry and Technology Prague, Czechia, is pioneering advanced methods for detecting pesticide residues in challenging food matrices like chili peppers.

In this webinar, he will show how comprehensive two-dimensional gas chromatography coupled to mass spectrometry (GC×GC-MS), combined with QuEChERS extraction, can overcome the challenges posed by chili peppers — a matrix rich in capsaicinoids and other lipophilic compounds that often interfere with conventional GC-MS/MS analysis.

Dr. Stupák will share practical insights from his research, including method development and validation according to SANTE/11312/2021 (V2), achieving recoveries between 70–120 %, robust precision, and low µg/kg limits of quantification — even in highly complex samples. Real-sample analysis demonstrated multiple pesticide residues in all chili peppers tested, with dried samples showing higher contamination levels than fresh.

This session is ideal for food safety, analytical chemistry, and regulatory compliance laboratories seeking practical, reliable solutions for pesticide residue analysis in challenging matrices, and for anyone looking to improve confidence in their routine testing workflows using advanced GC×GC-MS techniques.